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Antibodies Against Positive Ev Markers, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Identification of intestine epithelial cell-derived Evs (I-Evs). Extracellular vesicles were isolated from murine intestinal tissues and digested by standard procedures. A , B The morphology and diameter of I-Evs were analysed by electron microscopy and Nanoparticle tracking analysis. C 200 μg I-Evs were placed onto different concentrations of sucrose solution, and analysed by western blot with an anti-CD63 antibody. D 40 µg of intestinal lysates and I-Evs were separated by SDS-PAGE and transferred to a PVDF membrane. β-Actin, CD63, TSG101, A33, <t>GRP94,</t> and TGF-β1 were detected using antibodies. All data were verified by three independent experiments
Grp94, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Identification of intestine epithelial cell-derived Evs (I-Evs). Extracellular vesicles were isolated from murine intestinal tissues and digested by standard procedures. A , B The morphology and diameter of I-Evs were analysed by electron microscopy and Nanoparticle tracking analysis. C 200 μg I-Evs were placed onto different concentrations of sucrose solution, and analysed by western blot with an anti-CD63 antibody. D 40 µg of intestinal lysates and I-Evs were separated by SDS-PAGE and transferred to a PVDF membrane. β-Actin, CD63, TSG101, A33, <t>GRP94,</t> and TGF-β1 were detected using antibodies. All data were verified by three independent experiments
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Identification of intestine epithelial cell-derived Evs (I-Evs). Extracellular vesicles were isolated from murine intestinal tissues and digested by standard procedures. A , B The morphology and diameter of I-Evs were analysed by electron microscopy and Nanoparticle tracking analysis. C 200 μg I-Evs were placed onto different concentrations of sucrose solution, and analysed by western blot with an anti-CD63 antibody. D 40 µg of intestinal lysates and I-Evs were separated by SDS-PAGE and transferred to a PVDF membrane. β-Actin, CD63, TSG101, A33, <t>GRP94,</t> and TGF-β1 were detected using antibodies. All data were verified by three independent experiments
Anti Grp94 Antibodies, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+grp94/HSP90B1+Human%2C+Heat+Shock+Protein+90kDa+Beta+(GRP94)+Member+1+Human+Recombinant+Protein%2C+HEK/pmc09586796-75-23-30
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Identification of intestine epithelial cell-derived Evs (I-Evs). Extracellular vesicles were isolated from murine intestinal tissues and digested by standard procedures. A , B The morphology and diameter of I-Evs were analysed by electron microscopy and Nanoparticle tracking analysis. C 200 μg I-Evs were placed onto different concentrations of sucrose solution, and analysed by western blot with an anti-CD63 antibody. D 40 µg of intestinal lysates and I-Evs were separated by SDS-PAGE and transferred to a PVDF membrane. β-Actin, CD63, TSG101, A33, <t>GRP94,</t> and TGF-β1 were detected using antibodies. All data were verified by three independent experiments
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Identification of intestine epithelial cell-derived Evs (I-Evs). Extracellular vesicles were isolated from murine intestinal tissues and digested by standard procedures. A , B The morphology and diameter of I-Evs were analysed by electron microscopy and Nanoparticle tracking analysis. C 200 μg I-Evs were placed onto different concentrations of sucrose solution, and analysed by western blot with an anti-CD63 antibody. D 40 µg of intestinal lysates and I-Evs were separated by SDS-PAGE and transferred to a PVDF membrane. β-Actin, CD63, TSG101, A33, <t>GRP94,</t> and TGF-β1 were detected using antibodies. All data were verified by three independent experiments
Human Grp94 Protein Raybiotech, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Identification of intestine epithelial cell-derived Evs (I-Evs). Extracellular vesicles were isolated from murine intestinal tissues and digested by standard procedures. A , B The morphology and diameter of I-Evs were analysed by electron microscopy and Nanoparticle tracking analysis. C 200 μg I-Evs were placed onto different concentrations of sucrose solution, and analysed by western blot with an anti-CD63 antibody. D 40 µg of intestinal lysates and I-Evs were separated by SDS-PAGE and transferred to a PVDF membrane. β-Actin, CD63, TSG101, A33, GRP94, and TGF-β1 were detected using antibodies. All data were verified by three independent experiments

Journal: Molecular & Cellular Toxicology

Article Title: Intestine epithelial cell-derived extracellular vesicles alleviate inflammation induced by Clostridioides difficile TcdB through the activity of TGF-β1

doi: 10.1007/s13273-022-00280-8

Figure Lengend Snippet: Identification of intestine epithelial cell-derived Evs (I-Evs). Extracellular vesicles were isolated from murine intestinal tissues and digested by standard procedures. A , B The morphology and diameter of I-Evs were analysed by electron microscopy and Nanoparticle tracking analysis. C 200 μg I-Evs were placed onto different concentrations of sucrose solution, and analysed by western blot with an anti-CD63 antibody. D 40 µg of intestinal lysates and I-Evs were separated by SDS-PAGE and transferred to a PVDF membrane. β-Actin, CD63, TSG101, A33, GRP94, and TGF-β1 were detected using antibodies. All data were verified by three independent experiments

Article Snippet: Primary antibodies against CD63 (ab213090), TGF-β1 (ab8227), GRP94 (ab238126), TSG101 (ab125011), and β-Actin (ab8227) were purchased from Abcam (Cambridge, MA, USA).

Techniques: Derivative Assay, Isolation, Electron Microscopy, Western Blot, SDS Page, Membrane